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Registros recuperados: 3
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Identification and frequency of transposable elements in Eucalyptus Genet. Mol. Biol.
Bacci Jr.,Maurício; Soares,Rafael B.S.; Tajara,Eloíza; Ambar,Guilherme; Fischer,Carlos N.; Guilherme,Ivan R.; Costa,Eduardo P.; Miranda,Vitor F.O..
Transposable elements (TE) are major components of eukaryotic genomes and involved in cell regulation and organism evolution. We have analyzed 123,889 expressed sequence tags of the Eucalyptus Genome Project database and found 124 sequences representing 76 TE in 9 groups, of which copia, MuDR and FAR1 groups were the most abundant. The low amount of sequences of TE may reflect the high efficiency of repression of these elements, a process that is called TE silencing. Frequency of groups of TE in Eucalyptus libraries which were prepared with different tissues or physiologic conditions from seedlings or adult plants indicated that developing plants experience the expression of a much wider spectrum of TE groups than that seen in adult plants. These are...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Eucalyptus; Transposable element.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400019
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Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae) Neotropical Entomology
Silva-Pinhati,Ana C.O.; Bacci Jr.,Maurício; Siqueira,Célia G.; Silva,Aline; Pagnocca,Fernando C.; Bueno,Odair C.; Hebling,Maria J.A..
The isolation and maintenance of symbiotic basidiomycete fungi living in association with ants of the tribe Attini has been hindered by the slow growth rate of these fungi and the presence of other microorganisms on the surface of the material which the ants maintain inside their nests to provide a growth substrate for their symbiont. In this paper we describe a method which increases the efficiency of isolation of these fungal symbionts by over seven fold as compared to traditional isolation procedures. Underground nests of attine ants of the genera Atta, Acromyrmex, Trachymyrmex and Mycetarotes were located, from which samples containing the fungal symbiont and ants were collected and transported to the laboratory where the ants were able to clean the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atta; Acromyrmex; Trachymyrmex; Mycetarotes; Basidiomycete; Symbiosis.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100001
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Plant or fungal sequences? An alternative optimized PCR protocol to avoid ITS (nrDNA) misamplification BABT
Miranda,Vitor Fernandes Oliveira de; Martins,Vanderlei Geraldo; Furlan,Antonio; Bacci Jr.,Maurício.
The nuclear ribosomal DNA internal transcribed spacers (ITS1 and ITS2) from leaves of Drosera (Droseraceae) were amplified using "universal" primers. The analysis of the products demonstrated most samples were a molecular mixture as a result of unsuccessful and non-specific amplifications. Among the obtained sequences, two were from Basidiomycota fungi. Homologous sequences of Basidiomycota were obtained from GenBank database and added to a data set with sequences from Drosera leaves. Parsimony analysis demonstrated that one sequence was amplified from an Ustilaginomycetes fungus, and another from a Heterobasidiomycetes. Possibly these fungi were associated to leaves of Drosera, and not because of samples contamination. In order to provide optimization and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymerase; DNA; Drosera; Fungi; Phylogeny.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100018
Registros recuperados: 3
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